simca v.13 software Search Results


simca software  (Umetrics)
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Umetrics simca software
Simca Software, supplied by Umetrics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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simca p software  (Umetrics)
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Umetrics simca p software
Simca P Software, supplied by Umetrics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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package simca p  (Umetrics)
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Umetrics package simca p
Package Simca P, supplied by Umetrics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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package simca p v 13 0 3 0  (Umetrics)
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Umetrics package simca p v 13 0 3 0
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v 13 software  (SYSTAT)
96
SYSTAT v 13 software
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goat anti-rabbit igg antibody, peroxidase conjugated  (Merck KGaA)
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assays qiaamp dna stool mini kit qiagen  (Qiagen)
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gaba elisa  (ImmuSmol Inc)
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ImmuSmol Inc gaba elisa
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glutamate elisa  (ImmuSmol Inc)
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resource source identifier antibodies psd95 d27e11 xp rabbit mab cell signaling  (Cell Signaling Technology Inc)
97
Cell Signaling Technology Inc resource source identifier antibodies psd95 d27e11 xp rabbit mab cell signaling
Figure 4. Alterations of Brain Functions in AD-Recipient Mice (A) mRNA expression of myelin-related genes. (B) mRNA expression of different subunits of GABAA, GABAB, and NMDA receptors. (C and D) Quantification of GABA and glutamate in the frontal cortex. (E and F) Representative western blot <t>of</t> <t>PSD-95</t> <t>protein</t> in the frontal cortex and quantification by densitometry normalized to the loading control GAPDH. (G) mRNA expression of inflammatory cytokines, chemokines, and markers of microglial activation. n = 12 mice/group, #p = 0.09; *p < 0.05; **p < 0.01 versus CT-recipient mice, unpaired t tests, mean ± SEM (or SD for (C)–(E). FC, frontal cortex; St, striatum. CT-r, control-recipient; AD-r, AD-recipient. See also Figure S5.
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Image Search Results


Figure 4. Alterations of Brain Functions in AD-Recipient Mice (A) mRNA expression of myelin-related genes. (B) mRNA expression of different subunits of GABAA, GABAB, and NMDA receptors. (C and D) Quantification of GABA and glutamate in the frontal cortex. (E and F) Representative western blot of PSD-95 protein in the frontal cortex and quantification by densitometry normalized to the loading control GAPDH. (G) mRNA expression of inflammatory cytokines, chemokines, and markers of microglial activation. n = 12 mice/group, #p = 0.09; *p < 0.05; **p < 0.01 versus CT-recipient mice, unpaired t tests, mean ± SEM (or SD for (C)–(E). FC, frontal cortex; St, striatum. CT-r, control-recipient; AD-r, AD-recipient. See also Figure S5.

Journal: Cell reports

Article Title: Gut Microbiota-Induced Changes in β-Hydroxybutyrate Metabolism Are Linked to Altered Sociability and Depression in Alcohol Use Disorder.

doi: 10.1016/j.celrep.2020.108238

Figure Lengend Snippet: Figure 4. Alterations of Brain Functions in AD-Recipient Mice (A) mRNA expression of myelin-related genes. (B) mRNA expression of different subunits of GABAA, GABAB, and NMDA receptors. (C and D) Quantification of GABA and glutamate in the frontal cortex. (E and F) Representative western blot of PSD-95 protein in the frontal cortex and quantification by densitometry normalized to the loading control GAPDH. (G) mRNA expression of inflammatory cytokines, chemokines, and markers of microglial activation. n = 12 mice/group, #p = 0.09; *p < 0.05; **p < 0.01 versus CT-recipient mice, unpaired t tests, mean ± SEM (or SD for (C)–(E). FC, frontal cortex; St, striatum. CT-r, control-recipient; AD-r, AD-recipient. See also Figure S5.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PSD95 (D27E11) XP Rabbit mAb Cell signaling Cat#3450; RRID:AB_2292883 GAPDH (D16H11) XP Rabbit mAb Cell signaling Cat#5174; RRID:AB_10622025 Goat Anti-Rabbit IgG Antibody, Peroxidase Conjugated Merck Millipore Cat# AP132P; RRID:AB_90264 Chemicals, Peptides, and Recombinant Proteins Ampicillin sodium salt SIGMA-ALDRICH CAS Number: 69-52-3 Neomycin SIGMA-ALDRICH CAS Number: 1405-10-3 Metronidazole SIGMA-ALDRICH CAS Number: 443-48-1 Amphotericin B solubilized SIGMA-ALDRICH CAS Number:1397-89-3 Vancomycin hydrochloride VWR CAS Number: 1404-93-9 LPS Escherichia coli 0111: B4 SIGMA-ALDRICH Cat# LPS25 Critical Commercial Assays QIAamp DNA Stool Mini Kit QIAGEN Cat# 51504 Mouse Bio-Plex Pro Assays Biorad Customized Corticosterone ELISA kit Enzo Life Sciences Cat# ADI-900-097 GABA ELISA ImmuSmol Cat# BA E-2500 Glutamate ELISA ImmuSmol Cat# BA E-2400 Beta-hydroxybutyrate assay kit Abcam Cat# ab83390 Non-esterified fatty acids assay Randox Cat# FA115 Ethanol Colorimetric/Fluorometric Assay Kit Biovision Cat# K620-100 Deposited Data Raw data generated with the 16S rRNA gene sequencing SRA BioProject PRJNA553184 Experimental Models: Organisms/Strains Mouse C57BL/6J, specific pathogen-free (male) Janvier Labs N/A Oligonucleotides Primer sequences used for RT-qPCR See Table S3 N/A Software and Algorithms SPSS 25.0 IBM N/A R 3.4.2 Cran R project, https://cran.r-project.org/bin/ windows/base/old/3.4.2/ N/A USEARCH v10.02.240 Edgar, 2013 N/A Mothur v.1.25.0 Schloss et al., 2009 N/A BrainVoyager 20.6 Brain Innovation N/A MestReNova 10.0.2 Mestrelab Research N/A SIMCA-P+12.0 Umetrics N/A EthoVision XT software V14 Noldus N/A Leica Image Viewer Software 4.0.7 Leica Microsystems N/A ImageJ NIH, https://imagej.nih.gov/ij/download.html N/A ImageQuantTL 8.2 GE Heathcare N/A MetaboAnalyst V4.0 MetaboAnalyst, https://www.metaboanalyst. ca/ N/A GraphPad Prism 7 GraphPad Prism N/A e1 Cell Reports 33, 108238, October 13, 2020

Techniques: Expressing, Western Blot, Control, Activation Assay